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oct 1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology oct 1
    INS-1 β-cells were treated with D MSO or C ytokines (50 U/ml IL-1β + 150 U/ml IFNγ) for 16h or 24h ( A ), 16h ( B-E, H ), or 2-48h ( F-G ). A . Cells were processed for TUNEL analyses and mean ± SEMs of %apoptotic cells, relative to total cells, are presented. ( *,† Significantly different from corresponding DMSO group, p < 0.05; † significantly different from corresponding DMSO and cytokine groups, p < 0.05, n=3/group.) B-E, H . Representative blot from cells treated without or with P BA for iPLA 2 β, p65-NFκB, pPERK, and CHOP expression and corresponding densitometries. Tubulin <t>and</t> <t>Oct-1</t> (nuclear marker) were uses as loading controls in n=3 independent analyses. Cumulative densitometries are presented in . Representative blot of iPLA 2 β expression in cytosolic and nuclear fractions prepared, as described , and cumulative densitometry.
    Oct 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "ER Stress-Induced β-Cell Apoptosis is Linked to Novel Select Lipid Signaling at the Transcriptional Level: Implications in T1D Developmen t"

    Article Title: ER Stress-Induced β-Cell Apoptosis is Linked to Novel Select Lipid Signaling at the Transcriptional Level: Implications in T1D Developmen t

    Journal: bioRxiv

    doi: 10.64898/2026.03.02.708596

    INS-1 β-cells were treated with D MSO or C ytokines (50 U/ml IL-1β + 150 U/ml IFNγ) for 16h or 24h ( A ), 16h ( B-E, H ), or 2-48h ( F-G ). A . Cells were processed for TUNEL analyses and mean ± SEMs of %apoptotic cells, relative to total cells, are presented. ( *,† Significantly different from corresponding DMSO group, p < 0.05; † significantly different from corresponding DMSO and cytokine groups, p < 0.05, n=3/group.) B-E, H . Representative blot from cells treated without or with P BA for iPLA 2 β, p65-NFκB, pPERK, and CHOP expression and corresponding densitometries. Tubulin and Oct-1 (nuclear marker) were uses as loading controls in n=3 independent analyses. Cumulative densitometries are presented in . Representative blot of iPLA 2 β expression in cytosolic and nuclear fractions prepared, as described , and cumulative densitometry.
    Figure Legend Snippet: INS-1 β-cells were treated with D MSO or C ytokines (50 U/ml IL-1β + 150 U/ml IFNγ) for 16h or 24h ( A ), 16h ( B-E, H ), or 2-48h ( F-G ). A . Cells were processed for TUNEL analyses and mean ± SEMs of %apoptotic cells, relative to total cells, are presented. ( *,† Significantly different from corresponding DMSO group, p < 0.05; † significantly different from corresponding DMSO and cytokine groups, p < 0.05, n=3/group.) B-E, H . Representative blot from cells treated without or with P BA for iPLA 2 β, p65-NFκB, pPERK, and CHOP expression and corresponding densitometries. Tubulin and Oct-1 (nuclear marker) were uses as loading controls in n=3 independent analyses. Cumulative densitometries are presented in . Representative blot of iPLA 2 β expression in cytosolic and nuclear fractions prepared, as described , and cumulative densitometry.

    Techniques Used: TUNEL Assay, Expressing, Marker

    Related Articles

    Immunohistochemical staining:

    Article Title: CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REGγ
    Article Snippet: The proteasome inhibitor MG132 was purchased from Calbiochem (Germany). .. All antibodies were purchased as follows: Anti-CKIP-1 (sc-50225; for immunohistochemical analysis (IHC), 1:100 dilution; for immunofluorescent analysis (IF), 1:1000 dilution; for western blot analysis (WB), 1:500 dilution), anti-Oct-1 (sc-8024; for WB, 1:500 dilution; for immunoprecipitation analysis (IP), 1:50 dilution), anti-ABCG1 (sc-11150; for IF, 1:100 dilution; for WB, 1:200 dilution), anti-Lamin (sc-518013; for WB, 1:200 dilution), anti-SR-B (sc-32342; for IF, 1:100 dilution; for WB, 1:200 dilution), and anti-actin (sc-1616; for WB, 1:1000 dilution) antibodies were purchased from Santa Cruz. .. Anti-REGγ (ab157157; for WB, 1:500 dilution; for IP, 1:50 dilution; for IF, 1:100 dilution), anti-CD68 (ab125212; for IHC, 1:200 dilution; for IF, 1:200 dilution), anti-CD3 (ab16669; for IHC, 1:100 dilution), anti-SMA (ab9465; for IHC, 1:200 dilution), anti-ABCA1 (ab18180; for IF, 1:200 dilution; for WB, 1:200 dilution), and anti-ACAT-1 (ab168342; for WB, 1:500 dilution) antibodies were purchased from Abcam.

    Immunohistochemistry:

    Article Title: CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REGγ
    Article Snippet: The proteasome inhibitor MG132 was purchased from Calbiochem (Germany). .. All antibodies were purchased as follows: Anti-CKIP-1 (sc-50225; for immunohistochemical analysis (IHC), 1:100 dilution; for immunofluorescent analysis (IF), 1:1000 dilution; for western blot analysis (WB), 1:500 dilution), anti-Oct-1 (sc-8024; for WB, 1:500 dilution; for immunoprecipitation analysis (IP), 1:50 dilution), anti-ABCG1 (sc-11150; for IF, 1:100 dilution; for WB, 1:200 dilution), anti-Lamin (sc-518013; for WB, 1:200 dilution), anti-SR-B (sc-32342; for IF, 1:100 dilution; for WB, 1:200 dilution), and anti-actin (sc-1616; for WB, 1:1000 dilution) antibodies were purchased from Santa Cruz. .. Anti-REGγ (ab157157; for WB, 1:500 dilution; for IP, 1:50 dilution; for IF, 1:100 dilution), anti-CD68 (ab125212; for IHC, 1:200 dilution; for IF, 1:200 dilution), anti-CD3 (ab16669; for IHC, 1:100 dilution), anti-SMA (ab9465; for IHC, 1:200 dilution), anti-ABCA1 (ab18180; for IF, 1:200 dilution; for WB, 1:200 dilution), and anti-ACAT-1 (ab168342; for WB, 1:500 dilution) antibodies were purchased from Abcam.

    Western Blot:

    Article Title: CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REGγ
    Article Snippet: The proteasome inhibitor MG132 was purchased from Calbiochem (Germany). .. All antibodies were purchased as follows: Anti-CKIP-1 (sc-50225; for immunohistochemical analysis (IHC), 1:100 dilution; for immunofluorescent analysis (IF), 1:1000 dilution; for western blot analysis (WB), 1:500 dilution), anti-Oct-1 (sc-8024; for WB, 1:500 dilution; for immunoprecipitation analysis (IP), 1:50 dilution), anti-ABCG1 (sc-11150; for IF, 1:100 dilution; for WB, 1:200 dilution), anti-Lamin (sc-518013; for WB, 1:200 dilution), anti-SR-B (sc-32342; for IF, 1:100 dilution; for WB, 1:200 dilution), and anti-actin (sc-1616; for WB, 1:1000 dilution) antibodies were purchased from Santa Cruz. .. Anti-REGγ (ab157157; for WB, 1:500 dilution; for IP, 1:50 dilution; for IF, 1:100 dilution), anti-CD68 (ab125212; for IHC, 1:200 dilution; for IF, 1:200 dilution), anti-CD3 (ab16669; for IHC, 1:100 dilution), anti-SMA (ab9465; for IHC, 1:200 dilution), anti-ABCA1 (ab18180; for IF, 1:200 dilution; for WB, 1:200 dilution), and anti-ACAT-1 (ab168342; for WB, 1:500 dilution) antibodies were purchased from Abcam.

    Immunoprecipitation:

    Article Title: CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REGγ
    Article Snippet: The proteasome inhibitor MG132 was purchased from Calbiochem (Germany). .. All antibodies were purchased as follows: Anti-CKIP-1 (sc-50225; for immunohistochemical analysis (IHC), 1:100 dilution; for immunofluorescent analysis (IF), 1:1000 dilution; for western blot analysis (WB), 1:500 dilution), anti-Oct-1 (sc-8024; for WB, 1:500 dilution; for immunoprecipitation analysis (IP), 1:50 dilution), anti-ABCG1 (sc-11150; for IF, 1:100 dilution; for WB, 1:200 dilution), anti-Lamin (sc-518013; for WB, 1:200 dilution), anti-SR-B (sc-32342; for IF, 1:100 dilution; for WB, 1:200 dilution), and anti-actin (sc-1616; for WB, 1:1000 dilution) antibodies were purchased from Santa Cruz. .. Anti-REGγ (ab157157; for WB, 1:500 dilution; for IP, 1:50 dilution; for IF, 1:100 dilution), anti-CD68 (ab125212; for IHC, 1:200 dilution; for IF, 1:200 dilution), anti-CD3 (ab16669; for IHC, 1:100 dilution), anti-SMA (ab9465; for IHC, 1:200 dilution), anti-ABCA1 (ab18180; for IF, 1:200 dilution; for WB, 1:200 dilution), and anti-ACAT-1 (ab168342; for WB, 1:500 dilution) antibodies were purchased from Abcam.

    Transformation Assay:

    Article Title: Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region
    Article Snippet: Nuclear extracts (2 μg) were incubated with 1 μg of poly dI:dC (Sigma-Aldrich) and 32 P-labeled oligomers for 30 min in binding buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM DTT, 1 mM EDTA, and 5% glycerol) on ice. .. For the antibody-supershift assay, 5 μg of anti-Oct-1 (sc232X, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Oct-2 (sc233X, Santa Cruz Biotechnology), anti-GATA-3 (sc9009X, Santa Cruz Biotechnology), or anti- E26 transformation-specific protein 1 (Ets-1) (sc55581X, Santa Cruz Biotechnology) antibody was added to the mixture. ..

    Article Title: Transcription Factors Oct-1 and GATA-3 Cooperatively Regulate Th2 Cytokine Gene Expression via the RHS5 within the Th2 Locus Control Region.
    Article Snippet: Nuclear extracts (2 μg) were incubated with 1 μg of poly dI:dC (Sigma-Aldrich) and 32P-labeled oligomers for 30 min in binding buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mMDTT, 1 mM EDTA, and 5% glycerol) on ice. .. For the antibody-supershift assay, 5 μg of anti-Oct-1 (sc232X, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Oct-2 (sc233X, Santa Cruz Biotechnology), anti-GATA-3 (sc9009X, Santa Cruz Biotechnology), or anti- E26 transformation-specific protein 1 (Ets-1) (sc55581X, Santa Cruz Biotechnology) antibody was added to the mixture. ..

    Binding Assay:

    Article Title: A genome-wide analysis of DNA methylation identifies a novel association signal for Lp(a) concentrations in the LPA promoter
    Article Snippet: .. Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488) containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta ( CEBPB ) (Santa Cruz Biotechnology; sc-150x), anti-Oct-1 ( POU2F1 ) (Santa Cruz Biotechnology; sc232) plus anti-Oct-3/4 ( POU5F1 ) (Santa Cruz Biotechnology; sc5279) or anti-Nfatc1 ( NFATC1 ) as negative control (Santa Cruz Biotechnology; sc7294). .. Binding reactions with 3 × 10 5 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488).

    Article Title: A genome-wide analysis of DNA methylation identifies a novel association signal for Lp(a) concentrations in the LPA promoter
    Article Snippet: .. Binding reactions for super shift assays were performed for 30 min at 4–8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488) containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta ( CEBPB ) (Santa Cruz Biotechnology; sc-150x), anti-Oct-1 ( POU2F1 ) (Santa Cruz Biotechnology; sc232) plus anti-Oct-3/4 ( POU5F1 ) (Santa Cruz Biotechnology; sc5279) or anti-Nfatc1 ( NFATC1 ) as negative control (Santa Cruz Biotechnology; sc7294). .. Binding reactions with 3 × 10 5 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488).

    Negative Control:

    Article Title: A genome-wide analysis of DNA methylation identifies a novel association signal for Lp(a) concentrations in the LPA promoter
    Article Snippet: .. Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488) containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta ( CEBPB ) (Santa Cruz Biotechnology; sc-150x), anti-Oct-1 ( POU2F1 ) (Santa Cruz Biotechnology; sc232) plus anti-Oct-3/4 ( POU5F1 ) (Santa Cruz Biotechnology; sc5279) or anti-Nfatc1 ( NFATC1 ) as negative control (Santa Cruz Biotechnology; sc7294). .. Binding reactions with 3 × 10 5 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488).

    Article Title: A genome-wide analysis of DNA methylation identifies a novel association signal for Lp(a) concentrations in the LPA promoter
    Article Snippet: .. Binding reactions for super shift assays were performed for 30 min at 4–8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488) containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta ( CEBPB ) (Santa Cruz Biotechnology; sc-150x), anti-Oct-1 ( POU2F1 ) (Santa Cruz Biotechnology; sc232) plus anti-Oct-3/4 ( POU5F1 ) (Santa Cruz Biotechnology; sc5279) or anti-Nfatc1 ( NFATC1 ) as negative control (Santa Cruz Biotechnology; sc7294). .. Binding reactions with 3 × 10 5 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488).

    other:

    Article Title: Unique immune cell coactivators specify locus control region function and cell stage
    Article Snippet: Anti-Oct-1 (OCT1), rabbit polyclonal , Santa Cruz Biotechnology , Cat# sc-232; RRID: AB_2167065.



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    Image Search Results


    INS-1 β-cells were treated with D MSO or C ytokines (50 U/ml IL-1β + 150 U/ml IFNγ) for 16h or 24h ( A ), 16h ( B-E, H ), or 2-48h ( F-G ). A . Cells were processed for TUNEL analyses and mean ± SEMs of %apoptotic cells, relative to total cells, are presented. ( *,† Significantly different from corresponding DMSO group, p < 0.05; † significantly different from corresponding DMSO and cytokine groups, p < 0.05, n=3/group.) B-E, H . Representative blot from cells treated without or with P BA for iPLA 2 β, p65-NFκB, pPERK, and CHOP expression and corresponding densitometries. Tubulin and Oct-1 (nuclear marker) were uses as loading controls in n=3 independent analyses. Cumulative densitometries are presented in . Representative blot of iPLA 2 β expression in cytosolic and nuclear fractions prepared, as described , and cumulative densitometry.

    Journal: bioRxiv

    Article Title: ER Stress-Induced β-Cell Apoptosis is Linked to Novel Select Lipid Signaling at the Transcriptional Level: Implications in T1D Developmen t

    doi: 10.64898/2026.03.02.708596

    Figure Lengend Snippet: INS-1 β-cells were treated with D MSO or C ytokines (50 U/ml IL-1β + 150 U/ml IFNγ) for 16h or 24h ( A ), 16h ( B-E, H ), or 2-48h ( F-G ). A . Cells were processed for TUNEL analyses and mean ± SEMs of %apoptotic cells, relative to total cells, are presented. ( *,† Significantly different from corresponding DMSO group, p < 0.05; † significantly different from corresponding DMSO and cytokine groups, p < 0.05, n=3/group.) B-E, H . Representative blot from cells treated without or with P BA for iPLA 2 β, p65-NFκB, pPERK, and CHOP expression and corresponding densitometries. Tubulin and Oct-1 (nuclear marker) were uses as loading controls in n=3 independent analyses. Cumulative densitometries are presented in . Representative blot of iPLA 2 β expression in cytosolic and nuclear fractions prepared, as described , and cumulative densitometry.

    Article Snippet: The 1° antibodies for targeted proteins included: iPLA 2 β (sc-166616, 1:1,000), p65-NFκB (#sc-372, 1:1,000), CHOP (#sc-575, 1:1,000), IKBα (sc-371, 1:1000), tubulin (#sc-8035, 1:1,000), and Oct 1 (#sc-8024, 1:1,000) from Santa Cruz Biotechnology, Dallas, Texas; and pPERK (Cell Signaling Danvers, MA, #3179, 1:500).

    Techniques: TUNEL Assay, Expressing, Marker